It is often understood that our gut bacteria live off of the foods we eat. However, many gut bacteria can actually metabolize the mucous that protects the lining of our gut. In fact, many bugs have the ability to digest a specific sugar that is attached to our mucins, called sialic acid. Interestingly, some bacteria have the ability to cleave this sugar from the mucins, others have the ability to consume this sugar once it is released, and others have the ability to perform both of these tasks. Last week, a new method for how gut bacteria can transform sialic acid was discovered, that some bacteria can actually transform sialic acid before cleaving it, and that this may be clinically relevant for Crohn’s disease and colitis. The authors published their results in Nature Communications.

The authors were testing a common commensal bacteria, Ruminococcus gnavus, and noted that it had the ability to both cleave and consume sialic acid from gut mucins. When they identified the metabolites from this process they discovered that the sialic acid was actually being converted to a different form by these bugs. After further experimentation they realized that a type of enzyme, called an intramolecular trans sialidase, which had never before been observed in gut bacteria, was responsible. The researchers then compared the genes from R. gnavus to other bugs common in the gut and noted that a full 11% of human gut commensals had this enzyme, and that these bacteria were overrepresented in people with IBD. The authors think that the bugs who code for this enzyme have an inherent advantage over other gut microbiota because after they transform the sialic acid they can still use it for energy, whereas other bugs cannot, leaving the sugar all to themselves.

The paper did not discuss specific mechanisms as to why these bugs may be overrepresented in Crohn’s and colitis. They did however test a few molecules that inhibited the activity of the enzymes. Perhaps if these enzymes or the responsible bugs are the cause of IBD, than these inhibitors could be used as therapeutics to combat the disease.

We know that breastmilk is crucial to the development of a healthy infant’s microbiome. It contains many oligosaccharides that cannot be digested by the infant, and whose primary purpose appears to be stimulating the growth of specific microbiome bugs. There are, however, differences between new mothers’ milk. For instance, some mothers cannot produce 2′-fucosylated oligosaccharides, which are oligosaccharides that have a fucose sugar on the end. David Mills and his team at UC Davis recently investigated how the microbiomes of infants differed based on the presence or absence of fucosylated glycans in the milk that they drank. They published their work in the journal Microbiome last week.

Forty four infants who were fed breast milk had their microbiomes measured throughout the first 120 days of their lives. Thirty two of these infants were fed milk from woman with fucosylation ability (secretors), and twelve were from women without the fucosylation ability (non-secretors). When the researchers investigated the contents of the milk they found that it varied in many ways, besides fucosylation. For example, those women that did not fucosylate appeared instead to increase their monosaccharide sialylation, a sugar that has been linked to C. difficile infection. When the scientists compared the infants’ microbiomes in the two groups they discovered that secretor-fed infants achieved higher levels of Bifidobacteria and Bacteroides, and achieved these levels more quickly than non-secretor-fed infants. Instead, the non-secretor-fed infants had relatively higher levels of Enterobacteria, Clostridia, and Streptococci.

These differences may be important to the infants’ developments. For example, Bifidobacteria in the gut is associated with lower gut permeability and less inflammation. Also, Bifidobacteria and Bacteroides are large contributors to the production of short chain fatty acids and lactate, which have each been associated with gut health time and time again. A full 20% of the U.S. population is non-secretors, and it would be interesting to see if any epidemiologically significant differences exist between the two groups into adulthood. In either case, in the future it may be worth considering supplementing infant milk with fucosylated oligosaccharides if the lack of fucosylation does turn out to be detrimental to the baby.

A new way in which some gut bacteria rely on their hosts’ mucous for energy

Breastmilk varies between mothers – affects microbiome of infant